FIG 2 .

Validation of ISGs with antiviral potential. (A) Distribution of WNV infection in cells transduced with shRNA (normalized to the scrambled shRNA). Selected ISGs targeted by shRNA hits are labeled in blue. (B) Pie chart showing classification of top ISG hits, grouped by their known or putative functional categories (see Table S2 in the supplemental material). (C) Pie charts showing the breakdown of the 47 ISGs that were validated by viral yield assays. The chart on the left shows the number of ISGs in which silencing resulted in enhanced WNV production (relative to cells transduced with the scrambled shRNA) at different time points. The chart on the right shows the relative fold differences in viral yield associated with silencing of these ISGs (see also Table S3). (D) mRNA was harvested from cells transduced with the indicated shRNA or the scrambled shRNA control, and relative expression of the corresponding ISGs was determined by qRT-PCR (see also Fig. S1). The results are the averages of three independent experiments and are shown as means ± standard deviations. Statistical significance was determined by Student’s t test (***, P < 0.001; **, P < 0.01; *, P < 0.05).