Fig. 5.

Effects of NBMPR on the basolateral uptake of [³H]uridine in primary cultured rat Sertoli cells. Confluent monolayers of primary cultured rat Sertoli cells were provided with 40 nM [³H]uridine in the basolateral compartment. After 15 minutes, the cells were lysed, and the lysates were counted to determine cellular accumulation of [³H]uridine. The cells were exposed to concentrations of NBMPR sufficient to inhibit ENT1 uptake (100 nM), ENT2 and ENT1 (100 μM), or unlabeled uridine (5 mM). Control cells were not given NBMPR or unlabeled uridine. Each point represents an average (± half the range) of duplicate wells of Sertoli cells derived from a mixture of three rats. The height of each bar is 26.81 (control), 1.33 (5 mM uridine), 0.53 (400 nM NBMPR), and 0.34 (100 µM) fmol mm⁻¹ min⁻¹.