Fig. 9.

Inhibition of [³H]uridine transport by NRTI in primary cultured rat Sertoli cells. Confluent monolayers of primary cultured rat Sertoli cells were exposed to media in the basolateral compartment containing 40 nM [³H]uridine and either AZT (5 mM), ddI (5 mM), or tenofovir disoproxil fumarate (TDF; 1 mM). Control wells were not exposed to any NRTI drugs. Media from the apical compartments were counted to assess transepithelial transport of [³H]uridine after 15 minutes. Each bar represents an average (± S.E.) of triplicate wells of Sertoli cells derived from a mixture of three rats. The height of each bar is 27.52 (control), 11.52 (AZT), 9.51 (ddI), and 13.79 (TDF). *Statistically significant (P < 0.05) compared with control.