FIGURE 7.

Internal pH during spore germination and outgrowth. IpHluorin accumulated in the spores (from expression controlled by P_sspE) allows pH measurements from t = 0 to approximately 300 min. Expression of IpHluorin from P_ptsG allows calculation of the pHi from approximately 200 min. Data are from a representative example. (A) Germination and outgrowth of B. subtilis spores at an external pH = 6.4 with 25 mM KAc. (B) Germination and outgrowth of B. subtilis spores at an external pH = 6.4 with 80 mM KAc.