Table 3.
Oligonucleotides used in this study.
| Oligonucleotide | Sequence (5′–3′) | Remarks |
|---|---|---|
| IpHlu2010_FW | ATGGATTCAATAGAAAAGGTAAGCATGAGTAAAGGAGAAGAAC | Forward primer for IpHluorin |
| IpHlu148_RV | CGACunderbarGTCGACTTTATTTGTATAGTTCATCCATGCC | Reverse IpHluorin primer for pDG148 |
| IpHlu148A_FW | CCCunderbarAAGCTTAAGGAGGAAGCAGGTATGGATTCAATAGAAAAG | Forward IpHluorin primer for pDG148 |
| IpHlu1729A_FW | ACGCunderbarCCTAGGATGGATTCAATAGAAAAGGTAAGC | Forward IpHluorin primer for pSG1729 |
| IpHlu2010_RV | CCCunderbarAAGCTTTTATTTGTATAGTTCATCCATGCCATG | Reverse IpHluorin primer for pSG1729 |
| PptsG_FW | ACGCunderbarCCTAGGGAAAGTAAATAAGGAAAGTGTCAAC | 5′ end of PptsG |
| PptsG_IpHlu_RV | CATACCTGCTTCCTCCTTTTTTACTAGTCTGACCTTAC | 3′ end of PptsG |
| PptsG_IpHlu_FW | GTAAGGTCAGACTAGTAAAAAAGGAGGAAGCAGGTATG | 3′ of PptsG and 5′ of IpHluorin |
| PspoIIAA_FW | ACGCCunderbarCTAGGCCATAGCGGTTGTATTC | 5′ end of PspoIIA |
| PspoIIAA_IpHlu_RV | CATACCTGCTTCCTCCTTGATATGATCGGATAATGAGTGTTTC | 3′ end of PspoIIA |
| PspoIIAA_IpHlu_FW | GAAACACTCATTATCCGATCATATCAAGGAGGAAGCAGGTATG | 3′ of PptsG and 5′ of IpHluorin |
| PspoIIID_FW | ACGCunderbarCCTAGGCTGACCATTGAGATGAATAAAG | 5′ end of PspoIIID |
| PspoIIID_IpHlu_RV | CATACCTGCTTCCTCCTTAAAATGGATGTGAGAAGTGTGAAATGAG | 3′ end of PspoIIID and 5′ of IpHluorin |
| PspoIIID_IpHlu_FW | CTCATTTCACACTTCTCACATCCATTTTAAGGAGGAAGCAGGTATG | 3′ of PptsG and 5′ of IpHluorin |
| PsspE_FW | ACGCunderbarCCTAGGTGAACATTAATGCGAAAGCATTG | 5′ end of PsspE |
| PsspE_IpHlu_RV | CATACCTGCTTCCTCCTTCGGTCATTAGAATGTCCAG | 3′ end of PsspE |
| PsspE_IpHlu_FW | CTGGACATTCTAATGACCGAAGGAGGAAGCAGGTATG | 3′ of PsspE and 5′ of IpHluorin |
Underlined bases: restriction enzyme recognition sites; bases in italics: Shine–Dalgarno sequence; bold bases: start codon.