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. 2013 Jul;94(1):171–182. doi: 10.1189/jlb.1212659

Figure 6. TLR4 deficiency does not decrease caspase-1 activity in alcohol-fed mice in the cerebellum.

Figure 6.

WT (n=8 or 7) and TLR4-KO (n=8 or 13) mice were fed with a control (PF) or an EtOH diet for 5 weeks, respectively. TLR4, various inflammasome receptors (NLRP1, NLRP3, NLRC4), and Pannexin-1 (A) and the inflammasome adaptor ASC, the inflammasome effector procaspase-1, and pro-IL-1β (B) were assessed by real-time PCR from whole cerebellar RNA extracts, normalized to 18S. Inflammasome activity was evaluated by the caspase-1 colorimetric assay from whole cerebellar lysates (C). Bars represent mean ± sem (*P<0.05, relative to appropriate PF or WT-EtOH controls; #P<0.05, relative to appropriate WT-PF controls by the Kruskal-Wallis nonparametric test).