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. 2013 Jul;94(1):171–182. doi: 10.1189/jlb.1212659

Figure 7. IL-1ra, anakinra, treatment attenuates the effect of alcohol-feeding on caspase-1 activity and IL-1β production in murine cerebella.

Figure 7.

WT mice were fed with a PF or an EtOH diet for 5 weeks and received daily i.p. IL-1ra (anakinra: 25 mg/kg) or an equal amount of saline injections. Inflammatory cytokines (TNF-α, MCP-1, and pro-IL-1β) and procaspase-1 (A) were assessed by real-time PCR from whole cerebellar RNA extract, normalized to 18S. Proinflammatory cytokines, TNF-α (B), MCP-1 (C), and IL-1β (D) of whole cerebellar lysates were measured by specific ELISAs. Inflammasome activity was measured by the caspase-1 colorimetric assay from whole cerebellar lysates (E). Acetyl-HMGB1 of whole cerebellar lysates were analyzed by IP (F) and assessed by densitometry, using β-actin Western blot for loading control on a separate gel (G). The cerebellar lysate of one representative ethanol-fed, IL-1ra-treated mouse was applied for IgG control, using the same amount of protein. Bars represent mean ± sem (*P<0.05, relative to appropriate PF or saline treated-EtOH controls; #P<0.05, relative to appropriate saline-treated PF controls by the Kruskal-Wallis nonparametric test. PF-saline, n = 8; PF-IL-1ra, n = 5; EtOH-saline, n = 7; EtOH-IL-1ra, n=9).