Figure 4. Airway sensitization of mice with RSV-infected DCs elicited a Th2-skewed immune response upon a RSV infection challenge.
RSV-infected BMDC (2×105/mouse) were instilled into the airways of naïve mice and infected with live RSV (1×105 PFU/mouse), 7 days after BMDC instillation. Supernatants from RSV-restimulated lung draining LN cells were analyzed for cytokines by Bio-Plex (A). Lungs were taken 8 days after the additional RSV infection (15 days later, the DC-RSV instillation) and were stained with PAS (B) and assessed by qPCR for mucus genes (C). Data represent mean ± se from five mice/group. *P < 0.05.