Skip to main content
. 2013 Jul;94(1):5–15. doi: 10.1189/jlb.0412195

Figure 6. RSV-infected BMDC-induced allergen exacerbation is long-lived and does not need to be simultaneous.

Figure 6.

The BMDCs were infected with RSV (MOI=1.0) and instilled into naïve Balb/c mice. After 1 week, the mice were exposed to CRA (1 μg/mouse) by intranasal instillation with repeated instillations, 6 and 8 days later (Days 13 and 15 of the protocol). Twenty-four hours after final allergen exposure (Day 16), the animals were assessed for AHR (A) by methacholine challenge. Cont, Control. Draining LNs were prepared to single-cell suspension and restimulated with allergen (2 μg/ml) and 48-h culture supernatant assessed for cytokines by Bio-Plex analysis (B). Formalin-preserved lung tissue (left lobe) was assessed for mucus and goblet cell hypertrophy by PAS staining of histology sections (C). Data represent mean ± se from five mice/group. *P < 0.05.