Figure 9. εPS and εC1B synergize to recapitulate WT PKC-ε concentration.

(A) Cells were transfected with the indicated constructs and assayed as in Fig. 6. (B) Representative images showing red BIgG (upper); the same image represented in gray scale (lower). Asterisks designate phagosomes. (C) LI was calculated as in Materials and Methods (mean±sem of 80 total events/construct from three to four experiments). Significance was determined by ANOVA with Tukey's post-test; ***P < 0.001. Dotted line indicates no concentration. (C) Localization of a chimeric fragment consisting of εPSδC1AεC1B-GFP is equivalent to PKC-ε with respect to distribution (B) and intensity (C).