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. Author manuscript; available in PMC: 2013 Jun 18.
Published in final edited form as: Science. 2007 Jul 6;317(5834):130–132. doi: 10.1126/science.1142311

Fig. 2. MVP function in the interaction of airway epithelial cells with P. aeruginosa.

Fig. 2

(A) Whole-cell lysates from WT-CFTR cells (CFT1-LCFSN) that had been treated with either a scrambled siRNA sequence or MVP-specific siRNA were subjected to electrophoresis and immunoblot analysis with MVP-specific antibodies. Specific siRNA treatment reduced the level of MVP by >90%. (B) WT-CFTR cells were treated with scrambled or MVP-specific siRNA then left uninfected (Con) or infected for 15 min with P. aeruginosa PA01-V (PA01). Cell lysates were separated on discontinuous sucrose gradients. Raft fractions were precipitated and subjected to immunoblot analysis with MVP-specific antibodies. (C) Scrambled or MVP-specific siRNA-treated WT-CFTR cells were incubated with the indicated P. aeruginosa strains, and the amount of bacterial internalization was determined. The internalization of P. aeruginosa by cells treated with MVP-specific siRNA is plotted as a percentage of that obtained for scrambled siRNA-treated cells. Error bars represent SEMs. *P < 0.05, **P < 0.01 by a two-tailed t test comparison with internalization by scrambled siRNA-treated cells.