(A) Six hours after intranasal inoculation with ~1.5 × 107
P. aeruginosa strain PA01-V, WT and MVP−/− mice were killed (n = 9 WT, 12 MVP−/−), and the lungs were removed and dissociated. The internalized percentage of total bacteria found in the lung is plotted. Bars represent mean values. *P < 0.0001 by unpaired two-tailed t test. (B) The lung suspensions from the same mice used in (A) were analyzed for total bacterial load. The number of bacteria per gram of lung is plotted. Error bars represent standard error of the mean. *P < 0.01 by unpaired two-tailed t test. (C) Survival curves over 96 hours after intranasal inoculation with ~4 × 106 colony-forming units of P. aeruginosa strain PA01-V (n = 17 WT, 15 MVP−/−). Hazard ratio = 2.96, 95% CI 1.27 to 13.1; P = 0.018.