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Functional assay of the 5-FU–TPGS conjugate. (a) Effect of the 5-FU–TPGS conjugate on P-gp inhibition determined by the flow-cytometric assessment of the uptake of rhodamine 123 (RH123) in H460/TaxR cells. (b) Western blot for the detection of p53 expression in response to 5-FU and 5-FU–TPGS in KB-3-1 and H460/TaxR cells.
