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. 2013 Jun 11;4(3):e00609-12. doi: 10.1128/mBio.00609-12

FIG 2 .

FIG 2 

H. pylori 251 blocks the induction of IFN-β and IL-12 in murine bone marrow-derived macrophages stimulated with L. acidophilus. BMDMs were stimulated with either L. acidophilus NCFM or H. pylori 251 or prestimulated with L. acidophilus NCFM for 1 h prior to addition of Helicobacter pylori 251. (A) IFN-β and IL-12 were measured by ELISA in supernatants after 10 h of stimulation (L. acidophilus NCFM plus H. pylori 251 versus L. acidophilus NCFM alone; *, P < 0.01). Unstim., unstimulated. (B) RNA was extracted after 2, 4, 6, or 8 h of stimulation, cDNA was synthesized, and the expression of Ifn-β and Il-12 was analyzed by RT-PCR (L. acidophilus NCFM plus H. pylori 251 versus L. acidophilus NCFM alone; *, P < 0.01). (C) H. pylori 251 was added at various MOI (1:1, 1:2, 1:4, 1:8, and 1:16, H. pylori/BMDM ratio) to BMDMs prestimulated with L. acidophilus (1 h). RNA was extracted after 4 h, cDNA was synthesized, and the expression of Ifn-β and Il-12 was analyzed by RT-PCR. (D) BMDMs were prestimulated with L. acidophilus (L. acid. pre-incub.) for 1 h, 1.5 h, 2 h, 2.5 h, and 3.5 h prior to addition of H. pylori 251, RNA was extracted after 4 h, cDNA was synthesized, and the expression of Ifn-β and Il-12 was analyzed by RT-PCR (L. acidophilus NCFM plus H. pylori 251 versus L. acidophilus NCFM alone; *, P < 0.01). Data are means and SD for triplicate cultures and are representative of three independent experiments.