FIG 4 .

H. pylori redirects the endocytotic pathway of L. acidophilus. Phagocytic uptake of bacteria into early endosomes and lysosomes is shown. (A) BMDMs were exposed to red-labeled L. acidophilus NCFM for 2 h. Cells were either labeled with an EEA1 antibody for early endosomes or transfected with Lamp1-YFP for lysosomes. (B) As per panel A, with the addition of labeled or unlabeled H. pylori 251 (magenta). Cells were prelabeled with Lysotracker DND-26 to label lysosomes, incubated for 2 h, fixed, permeabilized, and stained with the EEA-1 antibody or anti-H. pylori serum for unlabeled H. pylori (HP). (C) Percentage of the total L. acidophilus NCFM located in the endosomal compartment or lysosomal compartment after 1 h and 2 h of incubation (with or without H. pylori 251). SEM are shown (*, P < 0.05; **, P < 0.01). (D) Magnified image of L. acidophilus NCFM (red) localized in lysosomes labeled with Lyso-DND26 (green) (2-h time point). Yellow indicates colocalization (white arrow) of signal or L. acidophilus NCFM located within the lysosomes. Data are representative of three independent experiments.