Table 2.
Effects of E. amylovora HEP and LEP cells on bacteriophage plaque appearance and efficiency of plating
| Family and phage isolate | Isolation hosta | Phage groupb | Plaque appearance (EOP)c |
Clear plaque formationsd: | |||||
|---|---|---|---|---|---|---|---|---|---|
| HEP |
LEP |
||||||||
| Ea110R | Ea29-7 | EaD-7 | Ea6-4 | Ea17-1-1 | EaG-5 | ||||
| Myoviridae | |||||||||
| ϕEa9-6 | Ea17-1-1 | 1* | T (0.5) | N (0) | T (0.1) | C (1.0) | C (1.0) | C (1.4) | LEP |
| ϕEa9-7 | Ea6-4 | 1* | T (1.0) | T (1.0) | T (0.2) | C (1.0) | C (1.4) | C (1.0) | LEP |
| ϕEa10-1 | Ea17-1-1 | 1* | T (0.3) | T (—) | T (0.1) | C (0.8) | C (1.0) | C (0.5) | LEP |
| ϕEa10-2 | Ea6-4 | 1 | T (0.5) | N (0) | T (0.4) | C (1.0) | C (1.9) | C (1.0) | LEP |
| ϕEa10-4 | EaG-5 | 1 | T (0.4) | T (0.6) | T (0.3) | C (0.6) | C (1.8) | C (1.0) | LEP |
| ϕEa21-2 | EaG-5 | 1 | T (1.0) | T (0.3) | T (0.8) | C (1.3) | C (1.5) | C (1.0) | LEP |
| ϕEa21-4 | Ea6-4 | 1 | T (1.2) | T (1.3) | T (0.7) | C (1.0) | C (1.2) | C (0.9) | LEP |
| ϕEa35-2 | Ea17-1-1 | 1 | T (0.3) | N (0) | N (0) | C (0.6) | C (1.0) | C (0.9) | LEP |
| Siphoviridae | |||||||||
| ϕEa10-5 | Ea110R | [2] | C (1.0) | C (0.5) | C (0.6) | C (1.6) | C (1.9) | C (2.5) | Both |
| ϕEa35-7 | Ea29-7 | 2 | C (0.8) | C (1.0) | C (0.9) | N (0) | T (—) | T (—) | LEP |
| Podoviridae | |||||||||
| ϕEa10-8 | Ea29-7 | 3A | C (1.5) | C (1.0) | C (1.0) | T (—) | T (—) | T (—) | HEP |
| ϕEa10-11 | Ea17-1-1 | 3A | C (0.5) | C (0.2) | C (0.3) | C (0.5) | C (1.0) | C (0.6) | Both |
| ϕEa31-3 | Ea29-7 | 3A | C (1.0) | C (0.9) | C (0.7) | N (0) | T (0.3) | N (0) | HEP |
| ϕEa46-2 | EaD-7 | 3B | C (1.3) | C (1.1) | C (1.0) | T (—) | T (—) | T (—) | HEP |
| ϕEa1(h)e | Ea110R | 3C | C (1.0) | C (2.3) | C (1.2) | C (2.6) | T (—) | C (3.2) | Both |
| ϕEa9-4 | EaG-5 | 5 | T (0.5) | N (0) | T (0.2) | C (0.3) | C (0.9) | C (1.0) | LEP |
| ϕEa51-4 | Ea29-7 | 6 | C (1.5) | C (1.0) | C (0.9) | T (—) | T (—) | T (—) | HEP |
| ϕEa31-7 | Ea29-7 | * | C (0.9) | C (1.0) | C (1.4) | T (—) | T (—) | T (—) | HEP |
| ϕEa31-8 | EaD-7 | * | C (1.5) | C (0.9) | C (1.0) | T (—) | T (—) | T (—) | HEP |
| ϕEa45-1B | Ea29-7 | * | C (0.8) | C (1.0) | C (1.0) | N (0) | T (—) | T (—) | HEP |
| ϕEa46-1A2 | EaD-7 | * | C (3.0) | C (2.5) | C (1.0) | T (—) | T (0.7) | T (—) | HEP |
Original bacterial isolation host, as published in reference 36.
Bacteriophages were placed into families and groups based on RFLP patterns and transmission electron micrographs (36). * denotes the sole use of real-time PCR to place the phage into the Myoviridae or Podoviridae (47); [2] indicates a presumptive Siphoviridae phage based on nondetection by real-time PCR (47).
Plaque appearance categories: C, clear; T, turbid/hazy; N, no plaques. EOP is the phage titer on the bacterial isolate divided by the phage titer on the isolation host.
General category of bacterial host, based on HEP or LEP status. —, plaques were present but could not be counted and distinguished due to their hazy appearance.
Ea1(h) was obtained from A. L. Jones (38).