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Cytochrome c (CytC) binding assay. Binding is expressed as the percentage of cytochrome c bound to stationary-phase cells of strain BL23 and derivatives after incubation with 150 μg ml−1 cytochrome c for 10 min at room temperature. Data represent the means and standard deviations from three independent experiments. Asterisks indicate significant differences (P < 0.05 by the unpaired Student t test) between the indicated strain and the control strain (BL23).
