Table 3.
Viability of M. avium subsp. paratuberculosis and M. smegmatis in samples from piles 1 and 2 after 250 days of composting
| Sample typeb | Survival in sample from pile 1/pile 2 at composting day showna |
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| 1 |
35 |
67 |
96 |
250 |
||||||
| M. aviumc | M. smegmatis | M. avium | M. smegmatis | M. avium | M. smegmatis | M. avium | M. smegmatis | M. avium | M. smegmatis | |
| Nylon bag | ||||||||||
| Control | −/− | −/− | −/NAd | −/NA | −/− | −/− | −/− | −/− | −/− | −/− |
| M. smegmatis | −/− | +/+ | −/NA | +/NA | −/− | −/+ | −/− | −/− | −/− | −/− |
| M. avium | +/+ | −/− | +/NA | −/NA | +/+ | −/− | +/+ | −/− | +/+ | −/− |
| LN | +/+ | −/− | +/NA | −/NA | +/+ | −/− | −/+ | −/− | +/+ | −/− |
| Plastic bag | ||||||||||
| Control | −/− | −/− | −/− | −/− | −/− | −/− | −/− | −/− | −/− | −/− |
| M. smegmatis | −/− | +/+ | −/− | +/− | −/− | −/+ | −/− | −/− | −/− | −/− |
| M. avium | +/+ | −/− | +/+ | −/− | +/+ | −/− | −/+ | −/− | +/+ | −/− |
| LN | +/+ | −/− | +/+ | −/− | +/+ | −/− | −/+ | −/− | +/+ | −/− |
Viability in all samples was detected by PCR with the primer sets for M. avium subsp. paratuberculosis and M. smegmatis shown in Table 1.
The control samples represent ground beef not inoculated with M. avium subsp. paratuberculosis. The M. smegmatis samples represent lab-cultured M. smegmatis inoculated into ground beef. The M. avium samples represent lab-cultured M. avium subsp. paratuberculosis inoculated into ground beef. LN, M. avium subsp. paratuberculosis-positive mesenteric lymph tissue.
M. avium = M. avium subsp. paratuberculosis throughout.
NA, no samples available for analysis from pile 1 at 35 days of composting.