Table 1.
Primer | Sequence (5′→3′)a |
---|---|
P1 | CGGGATCCGTCACCCGATACGAGCGTGGACA |
P2 | CGGACCACCACTGGACCCCGTCAGTACATTGAAGCTTTTG |
P3 | CGGACCACCACTGGAATTTTGCCAGTCCACCGTCACC |
P4 | TCCAGTGGTGGTCCGCAGC |
P5 | CCGCTCGAGGCCACCATTAAAACTGGGATGC |
The underlined letters denote the coding sequences of the restriction endonuclease sites; 15-bp segments of the overlapping regions are in boldface and underlined.