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. 2013 May;79(10):3234–3240. doi: 10.1128/AEM.00363-13

Table 1.

Oligonucleotide primers used for the construction of the fusion enzymes

Primer Sequence (5′→3′)a
P1 CGGGATCCGTCACCCGATACGAGCGTGGACA
P2 CGGACCACCACTGGACCCCGTCAGTACATTGAAGCTTTTG
P3 CGGACCACCACTGGAATTTTGCCAGTCCACCGTCACC
P4 TCCAGTGGTGGTCCGCAGC
P5 CCGCTCGAGGCCACCATTAAAACTGGGATGC
a

The underlined letters denote the coding sequences of the restriction endonuclease sites; 15-bp segments of the overlapping regions are in boldface and underlined.