. 2013 May;79(10):3234–3240. doi: 10.1128/AEM.00363-13

Table 2.

Kinetic parameters of the wild-type CGTase and two fusion enzymes^a

Enzyme	V_max (g · liter⁻¹ AA-2G/h · mg enzyme)	k_cat (h⁻¹)	l-AA		Soluble starch
Enzyme	V_max (g · liter⁻¹ AA-2G/h · mg enzyme)	k_cat (h⁻¹)	K_m (g/liter)	k_cat/K_m (liters/g · h)	K_m (g/liter)	k_cat/K_m (liters/g · h)	K_i (g/liter)
Wild type	0.021 ± 0.016	1.575	0.545 ± 0.022	2.88	1.107 ± 0.029	1.422	ND^b
CGTΔE-CBM_Amy	0.089 ± 0.009	6.675	0.501 ± 0.015	13.32	0.964 ± 0.022	6.924	18.3
CGT-CBM_Amy	0.135 ± 0.010	10.125	0.422 ± 0.018	23.99	0.771 ± 0.021	13.132	23.1

Each value represents the mean of three independent measurements, and the deviations from the mean were below 5%.

ND, not detectable.