Table 3.
Comparison of the reaction activities, AA-2G yields, and stabilities of the wild-type and fusion enzymes
| Enzyme | Relative activity (%)a |
||
|---|---|---|---|
| Cyclization (α-cyclodextrin-forming activity) | Hydrolysis (starch-degrading activity) | Disproportionation | |
| Wild type | 100 | 100 | 100 |
| CGTΔE-CBM>Amy | NDb | 186 ± 10 | 198.0 ± 1.0 |
| CGT-CBMAmy | ND | 236 ± 6 | 306.7 ± 0.8 |
a
The cyclization, hydrolysis, and disproportionation reaction activities for the wild-type CGTase were 165 ± 5, 8.3 ± 0.1, and 806 ± 8 U/mg, respectively, and were defined as 100% of the relative activity. Each value represents the mean of triple independent measurements, and the deviations from the mean were below 5%.
b
ND, not detectable.