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. Author manuscript; available in PMC: 2013 Jun 18.
Published in final edited form as: Free Radic Res. 2011 Jul 25;45(10):1124–1135. doi: 10.3109/10715762.2011.602073

Figure 3. Protein Cys-OH formation colocalizes with IQGAP1 at the leading edge during VEGF-stimulated EC migration.

Figure 3

A and B, HUVECs stimulated with VEGF were double-labeled with anti-IQGAP1 antibody (red) and Cys-OH probe DCP-Bio1 (green). White arrows point to the leading edge where Cys-OH and/or IQGAP1 localizes. In A (lower panel), cells were incubated with 10mM dimedone together with the Cys-OH probe to show the specificity of DCP-Bio1 labeling. In B, IQGAP1-depleted cell (white dotted line) and IQGAP1 expressing cell in IQGAP1 siRNA-transfected HUVECs were shown. Small white arrows point that depletion of IQGAP1 using siRNA reduces Cys-OH formation at lamellipodial plasma membranes. Bar scale indicates 10 μm. Bar graph represents the DCP-Bio1 fluorescence intensity at the leading edge in IQGAP1-depleted (−) cells, expressed as fold change from that in IQGAP1-positive (+) cells set as 1.0 in HUVECs transfected with IQGAP1 siRNA. Total of 24 cells each were counted in 11 randomly selected high power fields from 2 independent experiments. *P<0.05.