Figure 5. Trapping Cys-OH by dimedone inhibits VEGF-stimulated EC migration.

Effects of dimedone on VEGF-stimulated EC migration using wound scratch assay (A) and modified Boyden chamber assay (B). A, confluent monolayer of HUVECs were pretreated with dimedone (10 mM) or vehicle for 30 min, and scratch wound is induced in the presence of VEGF (50 ng/ml). Images were captured immediately (0 hr) and 24 hours after the wound scratch. The representative images were from 3 independent experiments. B, HUVECs were plated at 1×10⁵ cell suspension in growth arrest media with dimedone (10 mM) or vehicle onto the upper chamber of Transwell inserts. Chemotaxis was stimulated by 50ng/ml VEGF in the presence of 10mM dimedone or vehicle in the lower chamber. After 6 hours, migrated cells were measured. Bar graph represents averaged data, expressed as cell number counted per eight fields (× 200) and fold change over that in unstimulated cells (control). * p <0.05 vs. vehicle without VEGF.