Figure 5.

Effect of in vivo Siglec-5 expression of FVIII and VWF levels. C57B6 mice were injected hydrodynamically with an empty pLIVE plasmid of pLIVE-Siglec-5 (pLIVE-SG5), and after four days blood samples were taken to prepare plasma and livers were isolated. Liver sections of mice injected with pLIVE (A) or pLIVE-Siglec-5 (B) were analyzed for the expression of Siglec-5 using polyclonal anti-human Siglec-5 antibodies. Plasma samples were analyzed for levels of FVIII activity, VWF antigen and factor X (FX) activity (C). Levels of the various proteins in pLIVE-treated mice are relative to those in normal pooled plasma, which were arbitrarily set at 100%.