Table III.
Division kinetics of 1-cell mouse embryos cultured with different lots of mineral oil.
| Control | Lot 1 | Lot 2 | |
|---|---|---|---|
| Embryos | 42 | 42 | 42 |
| t2 (h) | 10.2 (9.8–10.7) | 10.7 (10.3–11.1) | 11.0 (9.9–12.2) |
| t3 (h) | 34.1 (33.4–34.7) | 35.6 (34.8–36.4)* | 35.2 (34.2–36.2) |
| t4 (h) | 34.9 (34.2–35.6) | 36.6 (35.7–37.5)* | 35.9 (34.8–37.0) |
| t5 (h) | 44.7 (43.8–45.5) | 48.4 (47.3–49.5)*** | 47.4 (45.7–49.1)*** |
| t8 (h) | 47.9 (46.6–49.3) | 51.8 (50.3–53.2)** | 49.7 (47.6–51.8) |
| tM (h) | 59.7 (58.1–61.2) | 65.4 (64.0–66.9)*** | 62.0 (60.1–63.9) |
| tB (h) | 73.1 (71.0–75.2) | 77.6 (75.1–80.1)* | 76.9 (73.7–80.1) |
| tEB (h) | 91.1 (88.4–93.7) | 97.1 (94.4–99.9)** | 94.3 (92.0–96.6) |
| cc2 (h) | 23.8 (23.4–24.2) | 24.9 (24.3–25.4) | 24.2 (22.8–25.6) |
| s2 (h) | 0.8 (0.6–1.1) | 1.0 (0.7–1.3) | 0.7 (.5–1.0) |
| i3 (h) | 9.8 (9.4–10.2) | 11.8 (11.1–12.4)*** | 11.4 (10.6–12.2)*** |
| s3 (h) | 3.3 (2.4–4.1) | 3.4 (2.7–4.0) | 2.8 (2.2–3.4) |
Values are mean (95% CI). t2, t3, t4, t5, t6, t7 and t8, cleavage times from a zygote to 2–8-cell embryo; tM, time to morula formation; tB, time to formation of blastocoel cavity; tEB, time to expanded blastocyst; cc2, duration of 2-cell stage (t3–t2); i3, duration of 4-cell stage (t5–t4); s2, synchrony second cell cycle (t4–t3); s3, synchrony of third cell cycle (t8–t5).
*P < 0.05, **P < 0.01, ***P < 0.001 (Dunnett's test).