FIG. 2.

Mechanical injury of the newt ventricle causes damage of the plasma membranes. The cell membrane-impermeable dye SYTOX Blue stains DNA of cells with damaged membranes. (A) Control hearts show no staining of cells in the ventricle (v), but signals in the atrium (a) and the aortic trunk (t), which is caused by preparation artifacts. (B) The right half of the ventricle contains numerous SYTOX Blue-labeled cells immediately after injury and one day later (C), indicating a high degree of damage. (D) Three days later, only a few SYTOX Blue-labeled cells remain. (E–F) After 7 dpi, no SYTOX Blue-labeled cells are present in the injured area anymore. Dashed lines separate the injured from the uninjured region; Arrows indicate SYTOX Blue-positive cells in the ventricle. Scale bar: 600 μm.