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. 2013 Feb 11;22(13):1921–1931. doi: 10.1089/scd.2012.0575

FIG. 3.

FIG. 3.

Increased deposition of extracellular matrix (ECM) during newt heart regeneration. (A, E) Control hearts display collagen III immunofluorescence around F-actin-positive cardiomyocytes and underneath the epicardium. Additionally, collagen III covers the ventricular lumen. (B, F) Increase of collagen III deposition at the outside of the injured region and around remaining cardiomyocytes at 1 dpi. (C, D, G, H, I, M) Collagen III deposits increase up to 35 dpi. Increased signal intensity is obvious around small F-actin-positive patches. (J, N) Reduced presence of collagen III after 84 dpi mainly in the outer myocardium. (K, O) After 120 dpi, collagen III levels decline with the exception of some deposits at the epicardium and the subjacent myocardium. (L, P) At 200 dpi, no significant differences to the control hearts are visible anymore. Arrows indicate the regions with higher collagen III levels at 84 and 120 dpi. F-actin: green; collagen III: red; DAPI: blue; scale bar: (A–D; I–L) 300 μm; (E–H; M–P) 50 μm.