Figure 5.

Effects of heme oxygenase-1 (HO-1) inhibition by a protein kinase C (PKC) inhibitor.

Notes: HuCC-T1 cells were treated with a single or combined application of 5-aminolevulinic acid ([ALA] 3 mM), celecoxib (10 μM), and PKC inhibitor (10 μM) for 4 hours in serum-free medium. (A) After 4 hours of irradiation, cell extracts were subjected to immunoblot analysis. The intensity of western band was obtained by calculating HO-1/β-actin using the Image J program (n = 3). (B) After irradiation, these cells were incubated for 24 hours in growth medium containing 10% fetal bovine serum. Cells were washed with phosphate buffered saline and stained with hematoxylin-eosin solution (n = 3). (C) Cells were washed with phosphate buffered saline, trypsinized, and counted (n = 6; data are represented as mean ± SEM). * denotes P < 0.05.

Abbreviations: ALA, 5-aminolevulinic acid; cele, celecoxib; HO-1, heme oxygenase-1; PKC, protein kinase C.