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. Author manuscript; available in PMC: 2014 Apr 26.
Published in final edited form as: J Proteomics. 2013 Feb 11;82:288–319. doi: 10.1016/j.jprot.2013.01.009

Table 4.

Transcriptional and proteoma analysis of D. rotundus Principal Submaxillary and Accessory glands.

Peptide link Total reads M * SP MW pI p A/P P/A Comments/Target 11 P 11 S 7 P 7 S
Putative secreted proteins

Enzymes

Phosphatase

BatTrinityAbyss-508800 1780 M * SIG 55.22 9.19 Y 0.42 2.36 Platelet function

Apyrase

BatTrinityAbyss-537653 3555 M * SIG 51.74 5.66 Y 0.67 1.49 Platelet function 1 12 1 12

DNAse (Deoxyribonuclease)

BatTrinityAbyss-499100 11477 M * SIG 33.91 6.06 Y 0.01 69.51 Neutrophil function
BatTrinityAbyss-532109 949 G * BL 36.22 5.81 N 1.18 0.84 Neutrophil function 1 15 1 14

Metalloprotease (ADAMTS-1)

BatTrinityAbyss-517665 10097 M * SIG 105.26 7.45 Y 0.01 74.75 Angiogenesis 0 1 8
BatTrinityAbyss-521171 6380 M * SIG 90.73 7.71 Y 2.34 0.43 Angiogenesis 1 1 ### 9

Serine protease

Plasminogen activator

BatTrinityAbyss-499018 1400766 M * SIG 44.42 8.78 Y 0 791.49 Fibrinolytic 1 1 1 13

Other serine proteases

BatTrinityAbyss-34346 381 S * SIG 27.93 9.24 Y 0.45 2.06 Chymase 1 16 1 16

Clotting pathways serine proteases

BatTrinityAbyss-499228 21684 N * CYT 16.93 6.15 Y 0.13 7.97 Protease ### 15 1 15
BatTrinityAbyss-489254 42256 M * SIG 30.95 5.8 Y 0.11 8.85 Protease

Dipeptidyl and tripeptidyl peptidases

BatTrinityAbyss-561424 2794 M * BL 87.86 5.65 Y 0.4 2.45 Dipeptidyl peptidases 1 6 1 7
BatTrinityAbyss-31529 825 M * SIG 52.1 6.32 1.13 0.87 Dipeptidyl peptidases 1 17 1 18

Legumains and cathepsins (may be lysosomal)

BatTrinityAbyss-529744 6521 M * SIG 44.33 6.19 Y 0.65 1.53 Cathepsin D 1 16 1 16
BatTrinityAbyss-32009 558 V * SIG 38.17 7.65 0.7 1.37 Cathepsin S 1 17 1 16

Other peptidases

BatTrinityAbyss-515689 12851 M * SIG 117.08 5.93 Y 0.53 1.89 Arginine convertase 1 7 1 7
BatTrinityAbyss-489052 2950 M * BL 107.07 5.73 Y 0.83 1.2 Aminopeptidase 1 7 1 8
BatTrinityAbyss-549050 2294 M * SIG 50.21 6.44 Y 0.25 3.96 Carboxy peptidases 1 11 1 11
BatTrinityAbyss-498793 4217 M * SIG 51.92 5.36 Y 0.87 1.14 Carboxy peptidases 1 11 1 11
BatTrinityAbyss-545838 1041 M * SIG 37.83 5.22 0.93 1.05 Prohibitin 1 13 1 14

Lipases

BatTrinityAbyss-549118 1863 M * SIG 51.34 5.79 Y 0.13 7.25 Sphingomyelinase ### 7 1 11
BatTrinityAbyss-39751 2505 M * SIG 62.08 6.1 Y 0.73 1.36 Carboxylesterase 1 10 1 2

Epoxide hydrolase

BatTrinityAbyss-508632 16840 M * SIG 52.4 6.79 1.04 0.96 Epoxide hydrolase 1 13 1 13

Other enzymes

BatTrinityAbyss-521692 816 M * CYT 33.42 7.77 0.87 1.12 Abhydrolase 1 16 1 16
BatTrinityAbyss-498378 2194 R * SIG 33.27 9.01 Y 0.78 1.26 Secreted protein 1 16 1 16

Protease inhibitor domains

Kunitz domain (TFPI-like)

BatTrinityAbyss-523646 386161 E * SIG 19.32 9.45 Y 0.03 36.3 Anticoagulant 0 1 14

Kunitz domain (Kunitz-type protease inhibitor)

BatTrinityAbyss-541822 3016 M * SIG 28.02 7.89 0.96 1.04 Kunitz inhibitor

Amyloid Kunitz protein

BatTrinityAbyss-507602 1896 M * SIG 85.08 4.69 Y 0.2 4.8 Mucin
BatTrinityAbyss-507612 13922 M * SIG 78.72 4.72 Y 0.2 4.98 Mucin

Collagen with VWB and Kunitz domains

BatTrinityAbyss-22377 4540 M * SIG 293.14 5.88 Y 0.87 1.15 Collagen 1 2 1 5
BatTrinityAbyss-555181 8671 M * SIG 118.27 7.75 Y 0.75 1.33 Collagen 1 5 1 6

Cystatin

BatTrinityAbyss-41885 1314 M * SIG 16.48 8.9 Y 2.26 0.44 Cysteine-type inhibitor

Kazal domain

BatTrinityAbyss-36340 45997 M * SIG 12.01 9.48 Y 4.91 0.2 Protease inhibitor 1 19 0

Serpins

BatTrinityAbyss-548577 2192 M * SIG 46.37 4.79 Y 0.61 1.61 Neuraserpin
BatTrinityAbyss-518161 1976 M * SIG 46.8 6.68 Y 0.33 2.93 α-1 antitrypsin 1 11 0
DrSigp-SigP-532391 2305 M * SIG 61.63 4.97 Y 0.72 1.38 Protease C1 inh. 1 7 1 8
BatTrinityAbyss-543253 3481 R * CYT 43.22 5.78 Y 1.86 0.54 Serpin 1 13 1 14
BatTrinityAbyss-25903 909 M * CYT 42.7 6.15 Y 0.71 1.38 Serpin 1 13 1 14

TIL domain containing protein

DrSigp-SigP-495835 342175 M * SIG 275.17 5.24 Y 4.98 0.2 Protease inhibitor 1 6 ### 16

Metalloproteinase inhibitor domain

BatTrinityAbyss-534229 862 M * SIG 24.18 9.01 0.84 1.16 Protease Inhibitor
BatTrinityAbyss-37180 425 M * SIG 23.04 8.58 0.74 1.29 Protease Inhibitor

Other protease inhibitors

BatTrinityAbyss-8258 1034 A * CYT 92.78 5.61 Y 1.39 0.71 α-Macroglobulin 1 8 1 8
DrSigp-SigP-210264 130 M SIG 18.17 8.73 1.24 0.73 Endopeptidase inhibitor
BatTrinityAbyss-321620 107 M SIG 25.5 9.51 1.36 0.65 Inter-α-trypsin inhibitor
DrSigp-SigP-36965 1046 M * SIG 15.45 7.76 Y 4.06 0.25 Peptidase inhibitor

Lipocalin and other lipid carriers

BatTrinityAbyss-466603 212065 P * SIG 21.18 5.09 Y 0 523.66 Lipocalin 0 1 17
BatTrinityAbyss-495622 1585466 M * SIG 24.32 7 Y 0 919.23 Lipocalin
BatTrinityAbyss-496761 1873290 M * SIG 24.35 7.04 Y 0 885.83 Lipocalin
BatTrinityAbyss-495626 2899256 K * SIG 23.08 8.92 Y 0 1104.63 Lipocalin 0 1 15
BatTrinityAbyss-495631 964371 M * SIG 22.44 4.92 Y 0 1089.84 Lipocalin
BatTrinityAbyss-495624 316695 M * SIG 23.89 4.74 Y 0 844.63 Lipocalin 0 1 15
BatTrinityAbyss-495634 268861 G CYT 18.05 9.71 Y 0 663.92 Lipocalin ### 1 1 15
BatTrinityAbyss-546328 16850 S * CYT 18.3 4.78 Y 0.01 66.03 Lipocalin 0 ### 17
BatTrinityAbyss-562380 1146 M * SIG 29 9.67 0.84 1.17 Lipocalin 1 15 1 15
BatTrinityAbyss-500440 1402119 M * SIG 6.3 3.76 Y 0 1214.13 Lipocalin
BatTrinityAbyss-509991 1206 P * CYT 29.41 8.86 Y 0.61 1.61 Lipocalin 1 16 1 16

Lipophilin/secretoglobin precursors

BatTrinityAbyss-500441 1614499 M * SIG 6.7 5.97 Y 0 1435.33 Antiinflammatory
BatTrinityAbyss-500442 2063350 M * SIG 8.98 4.08 Y 0 1273.86 Antiinflammatory 0 1 1
BatTrinityAbyss-515986 1309024 M * SIG 10.32 8.34 Y 0 484.86 Antiinflammatory 1 2 1 20
BatTrinityAbyss-497146 627793 L * SIG 11.05 8.68 Y 0 697.62 Antiinflammatory
DrSigp-SigP-515987 1090871 M * SIG 10.35 8.45 Y 0 451.73 Antiinflammatory
BatTrinityAbyss-515989 681163 M * SIG 5.76 5 Y 0 354.78 Antiinflammatory
BatTrinityAbyss-497133 163306 F * CYT 9.66 9.55 Y 0.02 46.27 Antiinflammatory ### 15 1 20
BatTrinityAbyss-497140 202043 S * CYT 12.08 9.69 Y 0.15 6.47 Antiinflammatory 1 15 1 20
DrSigp-SigP-515229 345027 M * SIG 6.26 6.03 Y 0 657.26 Antiinflammatory

Antigen 5/CRISP family

BatTrinityAbyss-495870 448565 M * SIG 27.39 8.79 Y 0.04 26.5 CRISP 1 16 1 16

Beta2-microglobulin/Class I major histocompatibility complex

BatTrinityAbyss-527040 10572 M * SIG 13.37 8.57 Y 0.71 1.41 MHC
BatTrinityAbyss-37556 341 S * CYT 19.81 6.27 Y 1.6 0.6 MHC

Neuropeptide/hormones

BatTrinityAbyss-500584 357219 M * SIG 20.58 6.44 Y 0 486.05 Vasodilator (PACA) ### 10 1 14
BatTrinityAbyss-538594 23548 M * SIG 12.78 ### Y 0 224.26 Vasodilator (CNP)

Extracellular matrix components

BatTrinityAbyss-41612 1466 R * SIG 20.25 9.16 Y 4.9 0.2 ECM 1 17 0
BatTrinityAbyss-67131 188 M SIG 19.38 5.65 0.58 1.51 ECM
BatTrinityAbyss-510471 1914 A CYT 86.72 6.29 0.98 1.02 ECM 1 3 1 3
BatTrinityAbyss-521514 4436 A CYT 330.47 5.58 Y 0.62 1.61 ECM ### 2 1 2

Lectins

BatTrinityAbyss-475358 289 N * CYT 26.53 8.89 1.17 0.81 Galectin-3 1 15 1 15
BatTrinityAbyss-36853 411 E * CYT 20.77 6.25 0.73 1.31 Galectin-7 1 19 1 19
BatTrinityAbyss-526622 3056 R * CYT 40.44 9 Y 0.8 1.25 Galectin-8 1 15 1 14
DrSigp-SigP-526623 1453 M * SIG 21.88 8.38 Y 0.69 1.43 Galectin-8
BatTrinityAbyss-467016 231 M * CYT 18.95 5.43 1.42 0.67 Galectin
DrSigp-SigP-534086 59576 M * SIG 26.66 7.77 Y 4.57 0.22 Lectin 1 18 1 18
BatTrinityAbyss-508153 13694 V * CYT 289.55 5.06 Y 0.35 2.87 Lectin ### 6 1 3

Antimicrobial peptides

Defensin

BatTrinityAbyss-401005 112 M * SIG 7.52 9.05 1.78 0.51 β-defensin

Lysozyme

BatTrinityAbyss-500942 1442243 M * SIG 16.63 9.34 Y 4.53 0.22 Lysozyme 1 19 1 19

Other antimicrobial agents

DrSigp-SigP-429618 105 M * SIG 12.51 5.65 0.59 1.35 BPI/LBP/CETP far
BatTrinityAbyss-86412 163 M * SIG 22.15 9.03 Y 1.73 0.54 lymphotoxin

Complement and regulators

BatTrinityAbyss-547414 1280 A * CYT 25.65 4.53 Y 0.51 1.92 Complement 1 16 1 16
DrSigp-SigP-483662 454 M SIG 43.51 6.21 0.95 1.02 Complement ### 7 ### 8
BatTrinityAbyss-483662 436 N SIG 43.03 6.44 0.87 1.1 Complement ### 7 #### 8
BatTrinityAbyss-563735 2587 R * SIG 129.72 6.1 Y 1.26 0.79 Complement 1 5 1 5
BatTrinityAbyss-40668 724 M * SIG 25.54 5.85 Y 0.4 2.35 Complement 1 17 1 16

Growth factors and immune regulators

BatTrinityAbyss-533465 1694 M * SIG 23.64 6.48 Y 0.3 3.26 Stromal cell factor 1 17 1 17
DrSigp-SigP-38368 1290 M * SIG 28.1 4.57 Y 1.23 0.8 hypothetical proteins 1 15 1 15

Immunity related products

C-C motif chemokine (CCL28-like)

BatTrinityAbyss-506850 4563 M * SIG 14.37 ### Y 0.03 30.37 Chemokine

T cell immunomodullatory protein

BatTrinityAbyss-511328 3228 M * BL 90.89 5.26 Y 0.65 1.52 T-cell antigen
BatTrinityAbyss-511332 23206 L * CYT 88.87 5.18 Y 0.8 1.25 T-cell antigen
BatTrinityAbyss-511331 23214 L * CYT 87.9 5.22 Y 0.8 1.25 T-cell antigen 1 7 1 7
BatTrinityAbyss-550694 2918 M * SIG 25.21 8.65 Y 3.99 0.25 Insulin secretion
BatTrinityAbyss-533680 338 M * SIG 49.25 7.76 Y 4.98 0.2 BPI/LBP/CETP 1 11 0

Other immunity related products (TSG-6)

BatTrinityAbyss-527888 8330 M * SIG 31.29 7.6 Y 0.02 42.67 TNF-inducible gene 0 1 14
BatTrinityAbyss-509950 844810 M * SIG 27.15 5.4 Y 0 345.53 TNF-inducible gene 1 15 1 15

MHC class I and II antigen

BatTrinityAbyss-507167 456 M SIG 31.63 5.76 Y 0.62 1.54 MHC class I B

Ig mu chain

BatTrinityAbyss-39685 1674 S * CYT 49.76 5.81 Y 1.57 0.63 IgG chain 1 9 1 9
BatTrinityAbyss-496807 479 M * SIG 15.15 9.12 Y 0.13 6.34 IgG chain ### 17 1 11
DrSigp-SigP-488712 579 M SIG 15.98 8.47 Y 0.65 1.48 IgG chain 1 11 1 11
BatTrinityAbyss-510560 570247 V * BL 51.47 5.46 Y 0.12 8.15 IgG chain 1 11 1 12
BatTrinityAbyss-138350 42 V CYT 13.54 9.43 0.36 1.37 IgG chain 1 10 1 11
BatTrinityAbyss-522329 586 V CYT 14.08 7.94 Y 0.67 1.44 IgG chain 1 11 1 11

Ig lambda chain

BatTrinityAbyss-505962 35933 L * SIG 24.36 7.04 Y 0.15 6.51 IgG chain 1 17 1 16
BatTrinityAbyss-46124 571 M SIG 15.93 5.21 Y 2.19 0.45 IgG chain
BatTrinityAbyss-521576 399 M SIG 15.31 5.07 Y 0.35 2.6 IgG chain
BatTrinityAbyss-505979 40645 T * SIG 26 8.17 Y 0.11 9 IgG chain 1 17 1 16

Mucins (hundreds not shown)

DrSigp-SigP-493041 6272756 M * SIG 182.75 5.29 Y 5 0.2 Mucin 1 2 1 1
BatTrinityAbyss-497247 1991177 M * SIG 13.25 4.55 Y 5 0.2 Mucin 1 15 1 18

M, indicates the presence of methionine.

SigP, presence of signal peptide.

MW, molecular weight of the the mature protein.

pI, isoelectric point.

*

, indicates the presence of stop codon.

A/P, ratio of transcripts present in the Acessory/Principal gland.

P/A, ratio of transcripts present in the Principal/Acessory gland.

p, p<0.05 for difference in expression levels for Principal vs Accessory glands.

11 P (Accessory Gland), 7 P (Principal Gland). Predict, predictive value for identification (1, maximum).

11 S (Accessory Gland), 7 S (Principal Gland). Slice from the 1D-gel (Figures 2A and 2B) where the protein was found in the proteome.

Inh., inhibitor. ECM, extracellular matrix.

SIG, signal peptide; CYT, cytosolic; BL, borderline prediction.