Table 2.
Ligand Protonation and Ferric Complex Formation Constants. Figures in parentheses give the uncertainty determined from the standard deviation between three independent titrations. pM values indicate the negative log value of the free iron(III) concentration at pH 7.4, 1 µM [Fe], 10 µM [L].
| log εmlh |
||||||
|---|---|---|---|---|---|---|
| Species | m l h | 3,4-LI (Me-3,2-HOPO) |
3,4-LI (Me-3,2-HOPO)2 (TAMmeg) |
3,4-LI (Me-3,2-HOPO) (TAMmeg)2 |
3,4-LI (TAMmeg) |
5-LIO (TAMmeg)2 (TAM) |
| LH | 0 1 1 | 8.41 (5) | 11.10 | 11.10 | 11.10 | 11.10 |
| LH2 | 0 1 2 | 15.03 (6) | 18.98 (2) | 22.20 | 22.20 | 22.20 |
| LH3 | 0 1 3 | 20.85 (6) | 25.48 (1) | 30.61 (2) | 33.30 | 33.30 |
| LH4 | 0 1 4 | 30.95 (3) | 37.32 (8) | 41.64 (3) | 40.89 (6) | |
| LH5 | 0 1 5 | 43.23 (6) | 48.75 (3) | 47.54 (1) | ||
| LH6 | 0 1 6 | 54.86 (3) | 52.85 (4) | |||
| FeL | 1 1 0 | 25.67 (2) | 30.65 (2) | 36.25 (2) | 43.78 (2) | 44.99 (6) |
| pM | 25.5 | 27.3 | 28.7 | 32.5 | 34.4 | |