Figure 2.
Arabidopsis TAH18 and DRE2 complement yeast dre2 when expressed together. (a) The indicated yeast strains were transformed with the listed plasmids. After growth for 40 h on liquid glucose-containing minimal medium, successive 10-fold dilutions of a cell suspension with OD600 nm = 0.5 were spotted on agar plates. Photographs were taken after 40 h of growth at 30 °C. (b) Isopropylmalate isomerase (Leu1) specific activities of cell extracts of yeast strains as in (a). Extracts were prepared from cells grown for 40 h on liquid glucose-containing minimal medium. —, no plasmid; Ø, empty plasmid.