Derivation of FSMCs from cultured mesothelium. Liver (a–a‴), spleen (b–b‴), kidney (c–c‴), lung (d–d‴), intestine (e–e‴), mesentery (f–f‴), diaphragm (g–g‴). Bright field (a–g), nuclear DAPI staining (a′–g′, h–n). Mesothelium-derived cells express α-SMA (a″–g″, h–h″), Vimentin (i–i″), FSP1 (j–j″), CD90 (k–k″), Collagen type I (l–l″), Collagen type IV (m–m″) and Fibronectin (n–n″). Merged images (a‴-g‴, h″−n″, DAPI is red, αSMA is green except h″, l″, m″). Original magnifications ×20 (a–n).