| Flow rate | Approximately 350–400 μl min−1 (back pressure should not exceed ~3,000 p.s.i. at 2% (vol/vol) B) |
| 85% (vol/vol) B | 0.0 min |
| 85% B to 30% B | 3.0 min |
| 30% B to 2% B | 12.0 min |
| 2% B | 15.0 min |
| 2% B to 85% B | 16.0 min |
| 85% | 23.0 min |
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