Table 4.
Validation of uniquely expressed genes induced by bleomycin in lung tissues from both Fra-1+/+ and Fra-1∆/∆ mice
|
Affymetrix ID |
Symbol |
Gene title |
Micro array |
qRT-PCR |
||
|---|---|---|---|---|---|---|
| |
|
|
WTT vs. WTC |
KOT vs. KOC |
WTT vs. WTC |
KOT vs. KOC |
| Fold change | Fold change | Fold change | Fold change | |||
| 10540085 |
Fbln2 |
Fibulin 2 |
2.28 |
ND |
2.35 ± 0.27* |
ND |
| 10531737 |
Hpse |
Heparanase |
ND |
1.98 |
ND |
1.57 ± 0.10* |
| 10587266 |
Gclc |
Glutamate-cysteine ligase, catalytic subunit |
ND |
1.70 |
ND |
1.58 ± 0.27* |
| 10509030 |
Runx3 |
Run related transcription factor 3 |
ND |
2.02 |
ND |
1.30 ± 0.14* |
| 10359697 |
Xcl1 |
Chemokine (C motif) ligand 1 |
ND |
2.15 |
ND |
2.57 ± 0.48* |
| 10531420 |
Cxcl11 |
Chemokine (C-X-C motif) ligand 11 |
ND |
2.53 |
ND |
4.72 ± 2.33* |
| 10590242 |
Ccr8 |
Chemokine (C-C motif) receptor 8 |
ND |
2.13 |
ND |
1.58 ± 0.00NS |
| 10574213 |
Ccl22 |
Macrophage-derived chemokine |
ND |
2.42 |
ND |
1.73 ± 0.54NS |
| 10576973 |
Col4a1 |
Collagen, type IV, alpha 1 |
1.96 |
ND |
1.83 ± 1.08NS |
ND |
| 10416230 |
Tnfrsf10b |
Tumor necrosis factor receptor superfamily, member 10b |
2.03 |
ND |
2.30 ± 1.09NS |
ND |
| 10357261 |
Marco |
Macrophage receptor with collagenous structure |
ND |
2.03 |
3.08 ± 0.11*† |
2.23 ± 0.21* |
| 10433776 | Snai2 | Snail homolog 2 (Drosophila) | ND | -1.92 | -2.23*† | -3.50 ± 1.95* |
Data are presented as mean ± SD (n=3-4). *P<0.05, PBS vs bleomycin of same genotype. NS means non significant. † Result did not agree with microarray result.