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. 2013 Jun 1;10:173. doi: 10.1186/1743-422X-10-173

Figure 1.

Figure 1

Optimization concentration of primer and probe: A: standard sample of WYMV as template. concentration of upstream, downstream primer and probe at a)200 nM 200 nM 200 nM; b) 200 nM 200 nM 400 nM; c) 100 nM 100 nM 200 nM. B: standard sample of β-actin as template. concentration of upstream, downstream primer and probe at a)200 nM 200 nM 200 nM; b) 200 nM 200 nM 400 nM; c) 100 nM 100 nM 200 nM. C: standard sample of 18S rRNA as template. concentration of upstream, downstream primer and probe at a)200 nM 200 nM 200 nM; b) 200 nM 200 nM 400 nM; c) 100 nM 100 nM 200 nM.