Skip to main content
. Author manuscript; available in PMC: 2013 Jun 18.
Published in final edited form as: Nat Biotechnol. 2011 Apr 13;29(5):443–448. doi: 10.1038/nbt.1862

Figure 1.

Figure 1

Hsa-miR-302b and/or hsa-miR-372 enhances reprogramming efficiency of human somatic cells. (a) Fold increase in number of human ESC-like colonies obtained per 15,000 cells compared to mock-transfected cells. Cells infected with 4Y ± miRNA were counted on day 21 after infection, whereas cells infected with 3Y ± miRNA were counted on day 31 after infection. *, a significant difference when compared to mock-transfected; P < 0.05; N = 6. Error bars represent mean ± s.e.m. (b) Expression of exogenous factors in iPSC lines that were picked and expanded after 3Y or 4Y infection and the indicated miRNA. Expression was determined by RT-qPCR using primers specific to only the exogenous factors. Data were normalized to BJ cells 3 d after retroviral infection with 4Y. (c) Expression of pluripotency markers in iPSC lines that were picked and expanded after 3Y or 4Y infection and the indicated miRNA. H9 hESCs shown as control. Expression was determined by RT-qPCR. Data were normalized to expression observed in BJ cells.