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. Author manuscript; available in PMC: 2013 Jun 18.
Published in final edited form as: Nat Biotechnol. 2011 Apr 13;29(5):443–448. doi: 10.1038/nbt.1862

Figure 2.

Figure 2

Hsa-miR-302b and hsa-miR-372 regulate expression of a number of targets that influence reprogramming of human somatic cells. (a) Heat map showing average expression from three independent experiments of 34 predicted targets of hsa-miR-302b and hsa-miR-372 on day 7 in the process of reprogramming. Expression was determined by qRT-PCR and was first normalized to GAPDH followed by normalization to mock-transfected cells. Statistically significant genes (P < 0.05; ANOVA) are labeled red. (b) Fold increase in reprogramming of human somatic cells infected with 4Y upon introduction of siRNAs against specific targets. Human ESC-like colonies were counted on day 21 after infection. N = 4. Error bars represent s.d. *, significant difference when compared to mock transfected; P < 0.05; measured by Kruskal-Wallis test. i, siRNA; ROCKi, ROCK inhibitor. (c ) Fold increase in reprogramming of human somatic cells infected with 3Y upon introduction of siRNAs against specific targets. Human ESC-like colonies were counted on day 31 after infection. N = 3. Error bars represent s.d. *, significant difference when compared to mock-transfected; P < 0.05; measured by Kruskal-Wallis test. i, siRNA; ROCKi, ROCK inhibitor.