Figure 4.

The midline-associated cells are early-born lineal descendants of dorsomedial (DM) lineages DM1 to DM3 and DM6. Flip-out clones induced at early L3 showing that no midline associated cells are produced by DM1 to DM3 and DM6 neuroblasts after this timepoint. However a substantial number of late secondary neurons is produced during the third larval instar. (A) DM1 flip-out clone, (B) DM2 flip-out clone, (C) DM3 and DM6 flip-out clone. Dotted circles in (A-C) indicate where midline associated cells would be located, but are absent. Scale bars, 25 μm.