Figure 5.

R45F08-Gal4 specifically labels the midline-associated neurons that innervate the fan-shaped body primordium in the late larval brain. (A)R45F08-Gal4 labels two bilateral groups of midline-associated cells as well as the fan-shaped body primordium (FBpr). The number and position of the labeled cell bodies, which project their neurites into the fan-shaped body primordium, correspond to the number and position of the midline cells revealed by Gal4^14-94 labeling (compare to Figure  2A; cells indicated by dotted line). No other neuronal structures are labeled implying that the labeled cell bodies give rise to the fan-shaped body primordium. (B) The R45F08-Gal4-labeled cells (green) are colabeled by the neuronal marker Elav (cyan). (B,B’) are two Z-projections taken at different focal planes. (C-H) Single sections taken at different depths show the R45F08-Gal4-labeled cells (green) in a brain hemisphere colabeled for Neurotactin (magenta) revealing the relative position of the primordium cell bodies (D-F), their neurite fascicles (F,G), and the fan-shaped body primordium (FBpr, (H)). Dorsomedial (DM) lineages DM1 to DM6 indicated with numbers. Circles in (G) indicate neurite fascicles. (I-I”)R45F08-Gal4-labeled cells (green) at the midline of a brain colabeled for DN-cadherin (magenta). In each hemisphere, the group of labeled cell bodies gives rise to four neurite fascicles that project into the fan-shaped body primordium (I”). One fascicle projects to the medial root of fan-shaped body (mrFB), one fascicle projects to the lateral root of the fan-shaped body (lrFB), and the remaining two fascicles project together to the dorsolateral root of the fan-shaped body (dlrFB); nomenclature according to [40]. (I-I”) are three Z-projections taken at different depths. Scale bars, 25 μm.