Figure 2. Transcriptional transactivation of p53-activated genes in response to virus infection requires p53-acetylation. (A) H1299 cells stably transfected with pcDNA, p53-WT or p53–8KR were infected with rVSV-GFP for the indicated times. Then, GFP expressing cells were selected by FACs sorting and Q-RTPCR analysis of the infected cells was performed. (B) MEFs derived from p53^−/− mice transiently co-transfected with GFP and pcDNA, p53-WT or p53–8KR at a ratio 1:10 were infected with VSV at MOI of 5 PFU/ml for the indicated times. Then, GFP expressing cells were selected by FACs and Q-RTPCR analysis was performed. The right parts represent the expression levels of p53 in each experiment.