Table 1.
Primer | Sequence 5’→3’ | References | PCR conditions * | |||
---|---|---|---|---|---|---|
Denaturing | Annealing | Extension | Cycles | |||
PLB | AGAGTTTGATCCTGGCTCAG | Kullen et al. (2000) | ||||
MLB | GGCTGCTGGCACGTAGTTAG | 94°C, 1 min | 50°C, 1 min | 72°C, 1 min | 30 | |
Bact 8 F | AGAGTTTGATCCTGGCTCAG | Edwards et al. (1989) | ||||
1391R | GACGGGCGGTGTGTRCA | Lane et al. (1985) | 94°C, 1 min | 56°C, 1 min | 72°C, 1 min | 30 |
16S/p2 | CTTGTACACACCGCCCGTC | Gürtler and Stanisich (1996) | ||||
23S/p7 | GGTACTTAGATGTTTCAGTTC | 94°C, 1 min | 56°C, 1 min | 72°C, 1 min | 35 | |
CS-f | GAGCTTGCTCCTCATTGATAA | Lee et al. (2004) | ||||
CS-r | TTGGATACCGTCACTACCTG | |||||
Uni-f | GATAAACCAAATGTGTAGGG | 94°C, 0.5 min | 58°C, 0.5 min | 72°C, 1 min | 35 | |
M13 | GAG GGT GGC GGT TCT | Huey and Hall (1989) | 94°C, 1 min | 45°C, 0.2 min | 72°C, 2 min | 40 |
RAPD1 | AGC AGG GTC G | Cocconcelli et al. (1995) | 94°C, 1 min | 29°C, 1 min | 72°C, 2 min | 20 |
RAPD2 | AGC AGC GTG G | 94°C, 0.5 min | 55°C, 0.5 min | 72°C, 0.3 min | 45 |
*All PCR programs included a first step of 94°C, 5 min and a final extension at 72°C, 5 min.