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mRNA expression of P450scc, StAR, and P450c17α (A); 5α reductase and 3α-HSD (B); and 17-βHSDIII and 3β-HSDVI (C) in Leydig cells of adult ALK3fx/fxCyp17cre+ and control mice. β-Actin was used as an internal control. Results are mean ± sem of RT-PCR analysis of different preparations of primary Leydig cells from at least six littermates. *, Significant differences (P < 0.05).
