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Effect of NR-INF-02 on nitric oxide release in unstimulated and lipopolysaccharide (LPS) stimulated RAW264.7 cells at 24 h of cell treatment (RAW264.7 cells were pre incubated at the indicated concentrations of NR-INF-02 for 30 min and treated with and without LPS for 24 h. After 24 h, the nitrite content in culture supernatant was analyzed by Griess reaction assay and expressed in μM. 1400 W dihydrochloride (12 μM) was used as reference control)
