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Simultaneous amplification in one vessel of Klebsiella pneumonia, Internal control (spiked IC) and 16S rDNA with optimized common annealing temperature (60° C) and specificity test of the PCR system designed for: (1) K. pneumonia (2) S. aureus (3) E. facium (4) P. aeruginosa (5) A. baumani (6) Human DNA (7) H2O. The bacterial concentration is (0.5 McFarland) of each strain. (M) Molecular Standard is indicated on the right
