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ELISA analysis of rhGH production. Different E. coli strains were transformed with plasmid containing sequence for rhGH and protein production was induced using IPTG. rhGH production was measured in supernatant and cell lysate using ELISA kits, trans: transformed, sup: supernatant, lys: lysate, LB: LB medium, 4YT: 4YT medium, T0: before induction and T5: 5h after induction.
