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Minimal membrane loss during preclearance at 600g. Shown is a Western blot analysis demonstrating minimal loss of membranes by preclearing at 600g. Preclearance (600g) and final (21,000g) pellets from the MCF protocol were analyzed for the PM protein PIN2. All lanes are derived from 6-mg root. Molecular mass markers (M, in kDa) and nonspecific bands (*) are indicated.
