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. Author manuscript; available in PMC: 2013 Jun 19.
Published in final edited form as: Integr Biol (Camb). 2011 Mar 24;3(5):578–591. doi: 10.1039/c0ib00141d

Fig. 4.

Fig. 4

The model recapitulated RA-induced feedback between BLR1 expression and MAPK activation. (A) Simulated BLR1 expression normalized to wildtype (WT) with Raf inhibition (−, 50% decrease in Raf initial condition) and overexpression (+, 50% increase in Raf initial condition) 48 h after the addition of RA. (B) Simulated phosphorylated Raf levels normalized to wildtype (WT) with BLR1 knockout (KO, BLR1 gene initial condition set to zero) and over-expression (+, 50% increase in BLR1 gene initial condition) 12 h after the addition of RA. (bottom panel) Corresponding model training data adapted from Wang and Yen.21 First row: effect of Raf siRNA (left) and overexpression (right) on the expression of BLR1 (Northern). Second row: effect of BLR1 knockout and overexpression of the level of phosphorylated Raf (S621).