Figure 1. Rad22-binding proteins involved in the HR pathway.

(a) Identification of Rad22-binding proteins. Immunoprecipitates were separated on SDS-gradient gels. The bands were visualized with CBB staining. Extracted proteins from each band were identified with LC-MS/MS. (b) Confirmation of in vivo interactions with Rad22. Co-precipitations with anti-FLAG antibodies were performed and immune-blotted with anti-GFP antibody to ensure their interaction between Rad22 and each protein. (c) Spontaneous HR frequency in cells overexpressing each gene. HR was measured by using the RDUX200 reporter as described in Materials and Methods. These experiments were performed three times and error bars represent the S.D. (d) Spontaneous Rad22 focus formation in cells overexpressing each gene. Cells were collected to count the number of GFP-positive cells. Error bars represent the S.D.