Figure 2. Association of HR frequency and IR sensitivity with bag101 expression.

(a) IR-induced HR frequency in bag101 overexpressing cells and the deletion mutants. Cells were irradiated with 250 Gy and 500 Gy, and the HR frequency was assayed immediately after irradiation. (b) IR-induced Rad22-foci in cells overexpressing Bag101 or containing Bag101 deletions. Cells were irradiated with 250 Gy and 500 Gy and collected 30 minutes later to count Rad22-focus positive cells. (c) IR sensitivity of each cell strain. Cells were irradiated with 250 Gy and 500 Gy, and immediately after irradiation, plated on YE plates to form colonies. All experiments in panels (a)–(c) were performed three times and the error bars represent the S.D. (d) The time course of H2A phosphorylation after IR exposure. Cells were irradiated with 500 Gy, and at indicated times after irradiation, the lysates from cells overexpressing bag101 (cropped) and deleting bag101 (cropped) were immunoblotted with anti-γ-H2A antibodies under the same condition as that from the wild type cell.