Skip to main content
. 2013 Jun 19;33(25):10405–10426. doi: 10.1523/JNEUROSCI.0014-13.2013

Figure 10.

Figure 10.

Effects of partial acetylcholine depletion in the dorsal striatum. A, Coronal corticostriatal slice showing the areas of stimulation and recording with histology and the typical location of AAV1-Cre-GFP. Inset: Tissue acetylcholine content in the dorsal striatum of sham-KO and vChAT-KO injected with AAV1-Cre-GFP. *p < 0.05, Student's t test. B, Locomotor ambulations in sham-KO and vChAT-KO mice after bilateral injections with AAV1-Cre-GFP. *p < 0.05, Student's t test; @p < 0.05, 2-way ANOVA. C, Locomotor ambulations in sham-KO and vChAT-KO mice after unilateral injections with AAV1-Cre-GFP. D, Representative traces (top) show the average responses of cortically evoked paired pulses before (left) and 5 min after bath application of amphetamine (right). Amphetamine reduced the eEPSC amplitude and increased the PPR in MSNs from saline-exposed, bilaterally injected sham-KO mice. E, F, In saline-exposed, bilaterally injected vChAT-KO mice, amphetamine reduced the eEPSC amplitude and increased the PPR in both GFP+ MSNs (E) and in nonfluorescent MSNs (F). G, Amphetamine increased the eEPSC amplitude and decreased the PPR in MSNs from amphetamine-treated, bilaterally injected sham-KO mice. H, Amphetamine reduced the eEPSC amplitude and increased the PPR in GFP+ MSNs from amphetamine-treated, bilaterally injected vChAT-KO mice. I, Amphetamine increased the eEPSC amplitude and reduced the PPR in nonfluorescent MSNs from amphetamine-treated, bilaterally injected vChAT-KO mice. Scale bars in A, 1 mm; DI, 100 pA, 5 ms.